Coenzyme Preference Of Streptococcus Pyogenes Delta(1)-Pyrroline-5-Carboxylate Reductase: Evidence Supporting Nadph As The Physiological Electron Donor

The streptococcal enzyme that catalyzes the last step in proline biosynthesis was heterologously expressed and the recombinant protein was purified to electrophoretic homogeneity and characterized thoroughly. As for delta(1)-pyrroline-5-carboxylate reductases from other sources, it was able to use either NADH or NADPH as the electron donor in vitro. However, with NADH the activity was markedly inhibited by physiological levels of NADP(+). Results also strengthen the possibility that an unusual ordered substrate binding occurs, in which the dinucleotide binds last.