Isolation And Molecular Identification Of T-2 Toxin-Producing Fusarium In Corn From Northeast China

Introduction. T-2 toxin belongs to a large group of trichothecene mycotoxins produced by various Fusarium molds. It is widely scattered in cereals and cereal, which can cause human and animal diseases. T-2 toxin has been paid more attention. The aim of this study was to isolate and identify T-2 toxin-producing Fusarium from moldy corn in Northeast China. Materials and Methods. A total of 20 corn samples from 10 corn field were collected. Isolation of the Fusarium was performed by RBM medium. T-2 toxin-producing with the corn medium and using HPLC detection. The highest T-2 production strains were identified by morphologic feature of macro-and microconidia and molecular method, using DNA sequence analysis. ITS region of ribosomal DNA was amplified and the PCR products were sequenced. Results. which 20 strains was selected for toxin-producting test. The 6th strain, which had the strongest capacity to produce T-2 toxin and homology with F. sporotrichioides reached to 100%. we can confirm the T-2 toxin-producing fungal was F. sporotrichioides.