Effect Of External Factors On The Curcumin/2-Hydroxypropyl-Beta-Cyclodextrin: In Vitro And In Vivo Study

The effect of 2-hydroxypropyl-beta-cyclodextrin (HP beta CD) on solubility, stability and oral bioavailability of curcumin by external factors adjustment, was investigated with an aim of a simple, stable and effective formulation. The phase solubility studies showed the solubility of curcumin increased slightly with increasing pH. However, the apparent stability constant (K (S)) were found to decrease with increasing pH from 1.29 x 10(4) M-1 at pH 3.0 to 5.22 x 10(3) M-1 at pH 7.0. The thermodynamic parameters were calculated for inclusion complex formation in aqueous solution. Interestingly, it could be concluded that the degrees of curcumin stability improved by HP beta CD grew with increasing drug-cyclodextrin binding ability. Furthermore, in vivo study not only revealed that the bioavailability of curcumin after oral administration to rats was significantly improved by curcumin/HP beta CD inclusion complex, but also showed more dramatic changes in the plasma concentration-time curve (1752.76-866.70 ng mL(-1) h) and the peak plasma concentration (370.10-178.11 ng mL(-1)) of drug by formation of complexes in pH 3-7 solution.