Molecular Mechanism Of Nf-Kappa B Signal Pathway In Autophagy Of Retinoblastoma Cell Line Hxo-Rb44

The pathogenic mechanism of retinoblastoma (RB) is still unclear. Abnormal cell autophagy is closely correlated with eye disease especially RB. This study thus investigated the role of cell autophagy disorder in the pathogenesis of RB and clinical implication. Using RB cell line HXO-RB44 cell as the model, UV irradiation was performed. Western blot measured autophagy condition of cells, as demonstrated by the expression level of related molecules and activation of signal pathways such as NF-kappa B. Autophagy inhibitor was used to treat HXO-RB44 cells, whose expression level of autophagy-related molecules and activation of NF-kappa B pathway were measured. NF-kappa B activator or inhibitor was further used for treatment, followed by measuring autophagy and NF-kappa B activation. The correlation between NF-kappa B signal pathway and RB pathogenesis was investigated. UV irradiation of RB cell line HXO-RB44 led to cell autophagy and NF-kappa B signal pathway activation. Inhibition of NF-kappa B pathway suppressed UV-induced autophagy of HXO-RB44 cells, whilst over-expression of NF-kappa B signal pathway enhanced UV-induced cell autophagy. The condition of NF-kappa B activation in RB tissues and their autophagy levels were closely correlated with disease severity. UV induces RB cell autophagy possibly via NF-kappa B signal pathway, suggesting the management of RB cell autophagy might be one strategy for treating RB.