The Clinical Significance And Contribution Of The Co-Stimulatory Molecule B7-H1 In Human Esophageal Cancer

Object: The study aims to investigate the clinical significance and contribution of the co-stimulatory molecule B7-H1 in human esophageal cancer tissues. Methods: Immunohistochemistry and western blot were used to analyze the expression levels of B7-H1 in human esophageal cancer tissues and cells. The associations between the expression of B7-H1 and other variables, including clinical parameters and prognosis, were explored using the fourfold and contingency table chi square tests respectively. After the expression of B7-H1 in human esophageal cancer cells was disrupted by an SiRNA, the proliferation and invasive activity of Eca-109 were tested using the CKK8 and Transwell assays respectively. Results: B7-H1 was highly expressed in esophageal cancer cells and tissues, and its expression level had a significant and positive correlation with the depth of tumor invasion (P < 0.05). The survival rate was much lower in patients with higher B7-H1 expression levels (P < 0.05). A B7-H1-knockdown model in Eca-109 cells was constructed by lentiviral transfection and RNAi, and downregulation of B7-HI remarkably inhibited the proliferation and invasion of esophageal cancer cells. Conclusion: Negative co-stimulatory molecule B7-H1 is regarded as an important bio-marker for the diagnosis and prognosis of esophageal cancer.