Calycosin Attenuates Tnbs-Induced Colitis Through Inibiting Inflammatory Cytokine And Oxidative Stress

Objective: To investigate the underlying protective effects of Calycosin on experimental colitis. Methods: The effect of Calycosin on pro-inflammatory genes mRNA expression and production were examined using real-time PCR and ELISA. Flow cytometry was used to determine the effect of Calycosin on LPS-induced ROS production in Raw264.7 cells. Western blot assay was used to determine the effect of Calycosin on NF-kappa B and MAPK pathway activation. For in vivo models, 2.5% TNBS was utilized through the catheter to induce experimental colitis. Body weight and DAI were evaluated every day. H&E staining were used to evaluate the effect on Calycosin on TNBS-induced colon damage. Moreover, MPO levels, MDA, GSH and SOD in TNBS-treated colon were determined by assay kits. Results: Calycosin significantly inhibited LPS-induced pro-inflammatory cytokines mRNA expression and production in Raw264.7 cells. 50 mg/kg Calycosin protect against TNBS-induced experimental colitis in mice, decreased oxidative stress and inhibited TNBS-induced inflammatory cytokine production, which is connected to the inhibition of NF-kappa B pathway. Conclusion: These findings revealed that Calycosin successfully ameliorated the effect of TNBS-induced colitis in mice.