Mir-206 Inhibits Cell Biology Of Human Glioblastoma By Targeting Met

In recent years, it has been reported that miRNA has been involved in the progression and development of some malignant tumors. The expression model and action mechanisms of miR-206 have not yet been reported in human glioblastoma (GBM). In the present study, we carried out miRNA mimics transfection, immunoblotting, and RT-PCR et al to investigate the function of miR-206 in GBM tissues and cells. In this work, our team identified that the expression of miR-206 was decreased in GBM tissues and cells, but increased in paired non-cancer tissues and NHA cells (all P<0.001). In addition, the expression level of MET mRNA and protein was increased in GBM tissues and cell lines (all P<0.001). Functionally, the ectopic miR-206 expression inhibited GBM cell proliferation, motility and invasiveness. Mechanically, we demonstrated that the 3' untranslated regions (3'-UTR) of MET was a bona-fond target of miR-206, and overexpressed miR-206 affected the post-transcription expression of MET protein in GBM cells. At the same time, ectopic miR-206 expression decreased the expression of EGFR, Bcl-2 and MMP2/9, and promoted the expression of Bax protein. In conclusion, miR-206 inhibits cell proliferation, migration and invasion by targeting 3'-UTR of MET in the development of GBM, thus miR-206 can serve as a tumor suppressor via targeting MET in the treatment of GBM. Therefore, miR-206-MET signals could be recommended as a potential target for treatment of GBM.