The Homing And Distribution Of Protection To Bone Marrow By Using Multidrug Resistance Gene 1 In Laryngocarcinoma Mice With Intensified Chemotherapy

Objective: The aim of this study is to observe the homing and distribution of the foreign multidrug resistance gene 1 (mdr1) in gene therapy. Method: After the bone marrow mononuclear cells of male BABL/C which was transferred with retrovirus- mediated mdr1 were transplanted into female Babl/c which was conducted radiotherapy with Co-60-gamma, the expression and distribution of foreign mdr1 in the bone marrow mononuclear cells, peripheral blood mononuclear cells and chief organs were detected by using immunohistochemistry, reverse transcriptase polymerase chain reaction (RT- PCR) and Fluorescence in Situ Hybridization (FISH), meanwhile, the homing of the bone marrow was monitored by immunohistochemistry and FISH. Result: There was no expression of external mdr1 in chief organs and tumor, P- glucoprotein expression percentage of the bone marrow mononuclear cells, peripheral blood mononuclear cells and tumor were respectively (9.58 +/- 1.24)%, (8.31 +/- 1.62)% and (48.52 +/- 11.42)% before performing chemotherapy, the homing rate of the bone marrow transferred with retrovirus-mediated mdr1 is 67.2%. Conclusion: The expression of the external mdr1 in bone marrow mononuclear cells and peripheral blood mononuclear cells was high, but no expression in the chief organs and tumors, the homing rate of the transplant of bone marrow transferred with retrovirus- mediated mdr1 was significantly increased.