Array Profiling Identified Dysregulated Mirnas And Target Genes And Pathways In Laryngeal Squamous Cell Carcinoma

Background: Laryngeal squamous cell carcinoma (LSCC) is the most common type of head and neck squamous cell carcinoma (HNSCC), and comprehensive post-transcriptional regulation is needed to be clarified. Objective: The purpose of this study was to investigate the miRNA basis of tumorigenesis in LSCC, and miRNA-gene-network and miRNA-pathway-network will be established with microarray analysis to reveal the molecular mechanism regulated by miRNAs. Methods: MicroRNA array and microarray were used to detect differently expressed miRNAs and mRNAs between the LSCC samples and para-carcinoma tissues collected from 2008 to 2013, then miRNA-mRNA network and miRNA-pathway network analysis were performed. Finally, the top dysregulated miRNAs were validated by quantitative real-time polymerase chain reaction (qRT-PCR). Results: 50 miRNAs (47 up-and 3 down-regulated), 2381 mRNAs (1542 up-and 839 down-regulated) were found to be differentially expressed. Bioinformatics analysis show that there were 31 different genes and 71 functional pathways regulated by the screened miRNAs. Hsa-miR-30a-5p expression level was validated by qRT-PCR. Conclusions: The research revealed tumorigenesis-related miRNAs, mRNAs, miRNA-mRNA, miRNA-pathway-network in LSCC. Hsa-miR-30a-5p could be considered as a potential diagnostic and therapeutic marker in LSCC.