Heat Shock Responses In Mice After Single Administration Of Quercetin

We investigated the effects of single dose quercetin (Q) on the responses to heat exposure in mice. Animals were exposed to heat under conscious conditions. Prior to the 2nd heat exposure mice were gavaged with Q (n=34) or vehicle (V, n=27). Tissue samples were collected after final heat exposure. Q‐treated mice had significantly lower plasma IL‐6 and IL‐12 levels, higher GSH/GSSG ratio and SOD, and lower 8‐isoprostane levels in plasma compared to V group. No significant differences in core temperature, blood pressure, and heart rate were observed between Q and V groups during heat exposure. However mice in the Q group that had a relatively high peak Tc during the 1st heat exposure showed a significant trend of having a lower peak Tc during the 2nd heat exposure. Overall, heat treatment groups had higher HSP72 and HSP90 protein levels in liver, heart and gastrocnemius muscle tissues compared to control group that were not exposed to heat (n=10). These results indicate single administration of Q produces anti‐inflammatory and antioxidant effects during moderate heat exposure without modulating HSF1 regulation of heat shock proteins. (supported by USUHS R091EH and ONR N0001411MP20025)