Telomerase Endometrial Cells Activity In Hyperplastic Processes

Objectives Endometrial hyperplastic processes (EHP) like a combination of stromal and glandular tissue components changes has properties both for self-healing, recurrence and for malignancy, depending on morphological changes degree with markers and predictors conducted research at cellular, molecular-genetic, immune-hystochemical levels. Telomerase activity estimation in endometrial cells at EHP. Methods According to WHO classification (1994) following groups were formed: 1 - simple hyperplasia -7 samples; II - complex hyperplasia -8; III - simple atypical hyperplasia -8; IV - complex atypical hyperplasia -7 specimens. The control groups with morphologically unchanged endometrium -11 samples: V group - proliferation phase - 6 specimens; VI group - secretion phase-5 samples. Relative telomerase activity was determined using real-time PCR with SYBR Green dye (RQ-TRAP) according to Wege et al. methodic. To improve the study accuracy we used from 4 to 6 cells samples and calculated their average value in each experiment. Results Women age was 45.38±1.66 years. In simple hyperplasia endometrial specimens, telomerase activity was 1.22±0.10a.u. (pI-IV Conclusions The parallelism presence between telomerase activity in approximately 85% of human tumors and telomerase reactivation in endometrial cells with complex atypical hyperplasia makes it possible to determine its activity fact like early malignancy marker.