Insulin-Like Growth Factor-1 Inhibits The Expression Of Autophagic Id1 In Cerebrovascular Endothelial Cells

Insulin-like growth factor (IGF)-1 has the effect of reducing blood glucose. However, it may cause obvious complications, which may be related to the autophagy related protein ubiquitin binding protein P62, inhibitor of DNA binding/differentiation 1 (ID1), or activated caspase-3. This study intends to discuss the protective role of IGF-1 on endothelial cells injured by autophagy and its impact on P62, ID1, and activated-caspase-3 expressions. IGF-1 was used to treat an endothelial cell autophagy injury model induced by hydrogen peroxide. Cell viability was assessed using an MTT assay. Cell autophagy and ID1 expression were detected by Western blot. IGF-1 is a protective molecule in endothelial cell autophagy injury. IGF-1 suppresses activated-caspase-3 expression and upregulates ID1 and P62 levels. IGF-1 contributes to cerebrovascular disease by inhibiting autophagy and promoting ID1 expression to antagonize endothelial cell autophagic death.