Fluorometric Determination Of Dna Using A Nucleic Acid Probe Ru(Bpy)(2)Pip(Vi)

A new ligand, 2-phenyl(5-bromo)-imidazo[f]-1,10-phenanthroline (PIP(VI) for short), and its coordination compound, Ru(bpy)(2)PIP(VI), were synthesized. The fluorescence spectrum of the interaction between Ru(bpy)(2)PIP(VI) and DNA was studied, and the intercalation binding between Ru(bpy)(2)PIP(VI) and DNA was proven. The maximum fluorescence intensity was produced in a solution with pH 7.45, with maximum excitation and emission wavelengths of 470 and 590 nm. The optimum conditions to determine DNA using Ru(bpy)(2)PIP(VI) were found. Under these conditions, the fluorescence intensity was proportional to DNA concentration. The linear range for calf thymus DNA is 4.8mug/Lsimilar to25 mg/L, and the limit of the detection for calf thymus DNA is 4.8 mug/L. The method is fast, simple, has high sensitivity and good selectivity, and has been satisfactorily employed in synthesized samples.