Up-Regulation Of Mir-10a And Down-Regulation Of Mir-148b Serve As Potential Prognostic Biomarkers For Osteosarcoma

Background: MicroRNAs (miRNAs) play key roles in cancer progression. The purpose of this study was to investigate miR-10a and miR-148b expression in osteosarcoma tissues and to further explored the prognostic value of the two miRNAs. Methods: Quantitative real-time PCR method was used to evaluate the expression levels of miR-10a and miR-148b in osteosarcoma (OS) tissues and adjacent non-tumor tissues. Furthermore, we investigated to clarify the relationship of miR-10a and miR-148 with clinicopathological features and survival in OS patients. Results: Our result suggested that miR-10a expression was up-regulated in OS tissues compared to adjacent non-tumor tissues. The expression level of miR-148b was down-regulated in OS tissues compared to adjacent non-tumor tissues. High expressions level of miR-10a and low expressions level of miR-148b were significantly association with advanced TNM stage, and metastasis (P<0.05). Furthermore, Kaplan-Meier survival analysis and log-rank test indicated that high expressions of miR-10a and low expression of miR-148b were correlated with shorter overall survival of OS patients (log-rank test, P<0.05). Multivariate Cox proportional hazards model showed that increased expression of miR-10a, decreased expression of miR-148b, TNM stage, and metastasis were independent prognostic markers of overall survival of patients. Conclusion: Our data showed that high miR-10a level and low miR-148b level were correlated with aggressive progression and poor prognosis of OS. These miRNAs may have clinical potentials as non-invasive diagnostic biomarkers for OS patients.