Evaluating The Photodynamic Biocidal Activity And Investigating The Mechanism Of Thiazolium Cyanine Dyes

In order to develop ideal photosensitizers for photodynamic therapy (PDT), a thiazolium group was introduced in cyanine dyes to possess the advantages of intense absorption in the visible region and anti-microbial activity. We evaluated the anti-bacterial activity of the three thiazolium cyanine dyes against Staphylococcus aureus (Gram-positive bacteria) and Escherichia coli (Gram-negative bacteria) in vitro. This is the first time that such kinds of cyanine dyes are applied in the photodynamic antibiotic area. Cyanines display excellent anti-bacterial activity to S. aureus and E. coli exposed to white light irradiation, and the corresponding light-induced biocidal efficacy of cyanines increased with irradiation time and concentration. At the same time, there was no observation of dark anti-bacterial abilities. Especially, cyanines show low cell cytotoxicity and excellent biocompatibility. These results suggest that thiazolium cyanine could work as a photosensitizer in PDT with great promise and broad applications for killing bacteria. Mechanistic studies suggest that the reactive oxygen species (ROS) is the vital factor for combating bacteria exposure to white light conditions, whereas it is not the only determining factor of the biocidal activity. The interaction of the cyanine to the cell membrane also plays a critical role in killing bacteria, which has exhibited a synergic effect of electrostatic and hydrophobic interactions. It influences the cell uptake and the membrane perturbation activity of the cyanines, which indirectly affects the biocidal activity.