Nanomatrix3d (R) Technology In Development Of Nanofibrouse Scaffolds: Biomedical Evaluation

Nanofiber based materials present unique properties favourable in many biomedical applications and electrospinning technics is the most common for their production due to its capability to produce fibrous materials with their structure and fiber diameters similar to those of extracellular matrix. In this research we evaluated in-vitro degradation, tissue responce and general toxicity of nanofibrose PLA and PCL 3D scaffolds produced by conventional method of electrospinning and using NanoMatrix3D (R) technology. There were no significant difference in mass loss between conventional and NM3D (R) electrospun scaffolds both during the static and dynamic degradation test, but dynamic condition significant facilitate degradation rate, that mimic in-vivo non-enzymatic hydrolysis. PCL and PLA scaffolds made by conventional method have shown toxic effect during the in-vivo tests due to residual chloroform that released with material degradation. NM3D (R) method allows clean scaffolds from residual solutions that makes it non-toxic and biocompatible. Subcutaneous, intramuscular and intraperitoneal implantation of PCL and PLA NM3D (R) electrospun nanofibrous scaffolds show their appropriate cell conductive properties, tissue and vessels formation in all sites. Thus, NM3D (R) PCL and PLA nanofibrouse electrospun scaffolds could be used in the field of tissue engineering, surgery, wound healing, drug delivery etc. due to their unique properties, nontoxicity and biocompatibility.