Regulatory Mechanism Of Asic1a Gene Silencing-Mediated Pi3k/Akt Signaling Pathway On The Proliferation, Apoptosis, Invasion And Migration Of Renal Cancer Cells

Objective: This study aimed to explore the regulatory mechanism of acid sensing ion channel 1a (ASIC1a) gene silencing-mediated PI3K/AKT signaling pathway on the progression of renal cancer cells. Methods: Human renal cancer 786-O cell line was separately transfected within five groups including blank group, negative control (NC) group, siRNA-ASIC1a group, LY294002 group and siRNA-ASIC1a + LY294002 group. mRNA and protein expressions were detected by qRT-PCR and Western blot. MTT assay, flow cytometry, transwell and scratch assays were performed to detect cell proliferation, apoptosis, invasion and migration, respectively. Results: Blank group and NC group showed no significant differences in related indexes (all P>0.05). Compared with blank group, ASIC1a expressions in siRNA- ASIC1a group and siRNA- ASIC1a + LY294002 group were significantly decreased (P<0.05), while it is not significantly different in LY294002 group (P>0.05). Compared with blank group, siRNA-ASIC1a group, LY294002 group and siRNA-ASIC1a + LY294002 group showed decreased mRNA and protein expressions of MMP-9 and Bcl-2 (all P<0.05) and phosphorylation of PI3K and AKT (all P<0.05), increased mRNA and protein expressions of Bax and caspase-3 (all P<0.05), a decreased cell proliferation, an increased cell proportion in G1 phase, a decreased cell proportion in S phase, an increased apoptosis rate, and decreased cell migration and invasion (all P<0.05), with significant differences. siRNA-ASIC1a + LY294002 group showed obvious differences in the changes of indexes (all P<0.05). Conclusion: ASIC1a gene silencing inhibited the activation of PI3K/AKT signaling pathway, in which way the proliferation, invasion and migration of renal cancer cells can be inhibited and their apoptosis promoted.