Changes In Platelet Gpib Alpha And Adam17 During The Acute Stage Of Atherosclerotic Ischemic Stroke Among Chinese

Glycoprotein (GP) Ib alpha ectodomain shedding has important implications for thrombosis and hemostasis. A disintegrin and metalloproteinase 17 (ADAM17) was identified to play an essential role in agonist induced GPIb alpha shedding. The relationship of GPIb alpha shedding and ADAM17 in the acute stage of atherosclerotic ischemic stroke (AIS) patients has not been thoroughly studied. A total of 306 patients and 230 controls matched for age, sex, race, history of hypertension and diabetes mellitus were enrolled in the study. GPIb alpha, ADAM17, glycocalicin were detected by flow cytometry, Western blotting, and enzyme-linked immunosorbent assay (ELISA) respectively. Compared with the control group, the expression of GPIb alpha in patients with acute ischemic stroke was significantly lower (P=0.000, P < 0.01). Plasma glycocalicin and ADAM17 in AIS group were higher than those in control group (P=0.699, P=0.000). Pearson's analysis showed glycocalicin bore no correlation with GPIb alpha in AIS patients (r=0.095, P > 0.05). GPIb alpha and National Institute of Health Stroke Scale (NIHSS) had negative correlation (r=-0.514, P < 0.01). Our findings indicate that ADAM17 may be a risk factor for ischemic stroke in Chinese and the expression of GPIb alpha can serve as a measure for stroke severity.