Renewable Fruit Wastes -Source For Production Of Levansucrase By Ssf Using Bacillus Megaterium Isolate

Bacterial Levansucrase (EC 2.4.1.10) is an extracellular enzyme produced by both Gram positive as well as Gram negative bacteria. Crude enzyme from Bacillus sp. rich in levansucrase can be used for the synthesis of levan, hence it can be considered as a satisfactory and promising producer of levansucrases. In present study fruit waste were used for production of Levansucrase enzyme to optimize the by using isolate of Bacillus megaterium. Three selective fruit wastes Banana peel, Orange peel and Lemon waste as substrates for different environmental conditions and production carried out by solid state Fermentation (SSF). Enzyme unit were calculated in both purified and crude form and confirmed by GOD method. Maximum production of enzyme was after 72 hrs of incubation at pH 6 and 50 degrees C temperature with orange peels as substrates in SSF.