Complete Genome Sequence Of A Recombinant Brassica Yellows Virus Infecting Nicotiana Tabacum

The entire genome of the recombinant brassica yellows virus Anhui (BrYV-AH) was isolated and characterized from tobacco (Nicotiana tabacum) leaves in the Anhui Province, China. Infected leaves were identified by leaf mosaic appearance, yellowing, and deformity. The BrYV-AH sequenced genome is comprised of 5,678 nucleotides (MF314820) and shares 93% nucleotide sequence identity with another brassica yellows virus isolate, the Chinese cabbage-Haidian (KF015269). The sequenced genome contains six open reading frames (ORFs) which encode putative proteins with functions in cell-to-cell movement and suppression of RNA silencing. Phylogenetic analysis places BrYV-AH alongside members of the genus Polerovirus, in the family Luteoviridae. Previous research has reported BrYV infection in tobacco, but the complete genome sequence of the virus had not yet been characterized. To the best of our knowledge, this is the ,first report of a complete recombinant BrYV genome sequence originating from a tobacco infection.