Impact Of Different Blood Transfusion Methods On The Expression Of Perforin And Granzyme B In Patients With Tumors

Objective: The impact of autologous and allogeneic blood transfusion on the postoperative expression of perforin (PFP) and granzyme (Gzm) in CD8+ T and natural killer (NK) cells was examined in patients with tumors. Methods: A total of 87 patients undergoing elective radical tumorectomy were divided into four groups: group I did not undergo blood transfusion; group II underwent allogeneic blood transfusion (ALBT); group III underwent hemodiluted blood autotransfusion (HBA); and group IV underwent preoperative autologous blood donation (PABD). The CD8+ T and NK cell percentages, as well as PFP and Gzm expression at different time points (before surgery (TO), postoperative 24 h (T1), 3 days (T2), 7 days (T3), and 10 days (T4)) in different groups were compared. Results: The number of NK cells in group II began decreasing at T1 (P<0.05) and reached a minimum level at T2; there was no significant difference in CD8+ T cells among the four groups. The levels of PFP and Gzm decreased at T1 in all groups (P<0.05), remained low in group II and IV (P<0.05), and returned to normal in group IV at T3. The levels of PFP and Gzm in CD8+ T cells returned to normal at T4, but the activity of NK cells remained low (P<0.05). Conclusions: Intraoperative ALBT can significantly inhibit the postoperative number and activity of toxic lymphocytes in patients, and ABD can reduce the occurrence of this damage.