Association Among Ana Patterns, Anti-Ena Antibody And Anti-Dsdna Antibody In Autoimmune Disease

To investigate the association among ANA patterns., anti-ENA antibodies and anti-dsDNA antibody in autoimmune diseases, and the reference significance of detecting these antibodies on the diagnosis and differential diagnosis of autoimmune diseases. Serum samples from 98 patients with autoimmune disorder were collected. ANA, anti-ENA and anti-dsDNA antibodies were detected using IIF, immuno-dot assay and ELISA. The distribution of different ANA nuclear types in the 98 samples was: pattern homogeneous(36/98, 36.74%); pattern nucleoplasm granular(41 /98, 41.84%); pattern nucleolar(11/98,11.22%); pattern nuclear membrane(1 /98, 1.02%); pattern centromere (1/98,1.02%); pattern homogeneous/nucleoplasm granular (2 /98, 2.04%)and pattern homogeneous/cytoplasm granular (6/98, 6.12%). These patterns may occur separately, also could appear at the same sample. Among these 98 samples with both ANA and anti-ENA positive, 42 cases were anti-dsDNA antibodies positive (42/98, 42.86%). In the 36 patients with ANA-homogeneous, 24 cases were anti-SSA antibody positive, 6 cases were anti-SSA/SSB antibody positive, and 6 cases were nRNP / Sm antibody positive. While in the 41 ANA-nucleoplasm granular samples, the positive rates of SSA, SSA/SSB and nRNP / Sm antibodies were 15.31%, 6.12% and 19.39%. One or several kinds of ENA antibodies could appear in patients with positive ANA and anti-dsDNA antibodies. The familiar nuclear types of ANA include pattern nucleoplasm granular, pattern homogeneous and pattern nucleolar. They occur individually or concurrently, which was correlated with the appearance of anti-ENA and anti-dsDNA antibodies. Combined Detection of ANA.. ENA and dsDNA antibodies has higher specificity, sensibility and accuracy in the diagnosis of autoimmune diseases, and is better in describing the correlation of the auto-antibodies appeared in patients and his clinical manifestation, thus provide valuable reference index to the diagnosis, differential diagnosis and therapy of AID.