Deciphering Circular Rna Axes In Early-Onset Breast Cancer Using A Breast Epithelial Risk-Progression 3d Culture Model

One of the known circular RNAs (circRNAs) functions is to sponge microRNAs (miRNAs). Studies reported dysregulated mRNA-circRNA-miRNAs axes as biomarker signatures in breast cancer (BC). Signatures in early-onset BC have not been characterized, while its incidence increased drastically worldwide. To address this gap, we performed circRNAs microarrays and miRNA-Sequencing on 3D breast epithelial risk-progression HMT-3522 S1 (S1) culture model to identify dysregulated post-transcriptional axes. S1 (non-neoplastic) cells form fully-polarized epithelium and closely mimic normal in vivo mammary epithelial morphology, while (pre-tumorigenic) Cx43KO-S1 (S1 silenced with shRNA for gap junction Cx43) lose epithelial polarity, multi-layer and mimic tumor-initiated in vivo mammary epithelial morphology. We detected through circRNA microarray (Arraystar Human circRNA Array V2) 75 significantly upregulated and 46 significantly downregulated circRNAs (Fold Change > 2, p-value Citation Format: Nataly Naser Al Deen, Nadia Atallah Lanman, Shirisha Chittiboyina, Sophie Lelievre, Heinrich Zu Dohna, Mounir AbouHaidar, Rabih Talhouk. Deciphering circular RNA axes in early-onset breast cancer using a breast epithelial risk-progression 3D culture model [abstract]. In: Proceedings of the Annual Meeting of the American Association for Cancer Research 2020; 2020 Apr 27-28 and Jun 22-24. Philadelphia (PA): AACR; Cancer Res 2020;80(16 Suppl):Abstract nr 278.