In Vitro Anthelmintic Study Of Bauhinia Acuminata Linn. Leaf Extracts Against The Housefly Worms

Bauhinia acuminataLinn. (Caesalpiniaceae) or its local name Dwarf White Orchid", "TapakKuda" or "Safed Kachnar" is a recent medicinal plant discovered which has been employed by the folks in treating of different types of ailmint. Therefore, the present study was made an attempt to investigate its anthelmintic quality against the housefly worms. The housefly worms were used as the experimental model due to its resemblance to parasitic pinworms. The advantages of this method are easy, economic, eco friendly, prominent and reproducible method compared to conventional method. Aqueous and ethanolic extracts with different concentrations (12.5, 25, 50, 100 and 200 mg/ml) were tested on the Missal domesticaworms in vitro assay. Determination of paralysis and death time was recorded. Albendazole was used as standard and distilled water as a control. From the preliminary phytochemical evaluation, tannins and saponins were detected in both extracts. In vitro anthelmintic assay revealed both extracts have shown significant effect in paralysis and death of the housefly worms in a dose dependent manner. In comparison of test solutions, ethanolic extracts was more effective than aqueous extract. As a conclusion, the leaves of Bauhinia acuminta could be used as an anthelmintic remedy, however, further studies were suggested to carry out in vivo using laboratory animals to confirm the safety efficacy, and toxicity profile of this plant. Further screening investigation is recommended to isolate the bioactive and total phytochemical compounds contribute to the anthelmintic property of this plant.