Isolation And Detail Characterization Of Abal T-Dna Insertion Mutant Line Of Arabidopsis Thaliana

Abscisic acid is a plant hormone that affects many biological processes during plant lifespan including stomatal movement, seed and bud dormancy and organ size. Nevertheless, the role of abscisic acid in photomorphogenesis remains rudimentary. To study the role of abscisic acid in different light conditions, the characterization of homozygous mutant lines in biosynthetic gene encoding zeaxanthin epoxidase (ABA1) is essential for genetic studies. Here, we present isolation and characterization of one of the T-DNA insertion mutant lines of Arabidopsis thaliana ecotype Colombia-0, where the insertion is located in 5' UTR region (SALK_027326). We identified the homozygous plants from a segregating parental line by PCR. The corresponding PCR products were identical to those of a confirmed homozygous line (SALK_027326C). However, the ABA1 transcript abundance in isolated homozygous line was almost identical to the ABA1 transcript abundance in the wild-type plants, as was revealed by RT-qPCR analysis. Because the phenotype of the selected homozygous line was also similar to wild-type plants in response to a low light, we conclude that the 5' UTR mutation in the ABA1 gene does not affect the gene function and thus this line is not an eligible candidate for further genetic studies.