Altered Long Noncoding Rna Profiles In Rat Ischemia-Reperfusion Injury After Ionps Administration

Background and aim: Iron oxide nanoparticles (IONPs) can protect hearts from ischemia reperfusion (I/R) injuries. Long noncoding RNAs (LncRNAs) have been believed to play important roles in regulation of many biological processes. The aim of our study was to investigate the lncRNA profile in I/R injury rat model following IONPs ferumoxytol administration. Methods: Models of I/R were induced by 30 min of ischemia followed by 24 hours of reperfusion in male Sprague-Dawley rats. The rats were randomized into three groups: sham group, I/R group, and ferumoxytol group. The profiles of lncRNAs and mRNAs were confirmed using Illumina RNA-Seq technology combining quantitative real-time PCR analysis. Results: Ferumoxytol leads to a significant reduction in the area of myocardial infarction. Iron deposition was observed around ischemic myocardial tissue by Prussian blue staining. Sequencing revealed the existence of a total of 19754 lncRNAs distributed in all chromosomes of the experimented rats. In ferumoxytol intra-myocardial injected heart, 433 lncRNAs were up-regulated, whereas 287 lncRNAs were down-regulated when compared with I/R injured hearts (fold-change > 2.0). In I/R injury hearts, 419 lncRNAs were up-regulated, whereas 393 lncRNAs were down-regulated when compared with sham heart (fold-change > 2.0). Top 10 lncRNAs were selected for RT-qPCR validation. Based on cis-acting target genes prediction and mRNA-sequencing results, NONRNOT219022-Reg3b is likely to be the key lncRNA-mRNA pair. Conclusion: We identified a set of lncRNAs aberrantly expressed in myocardial I/R injury rats with ferumoxytol, shedding lights for further research of the mechanism of feurmoxytol in myocardial protection and proposing a potential therapeutic target for I/R injuries.