Therapeutic Effects Of Acupuncture Treatment On Experimental Rats With Intracerebral Hemorrhage Via Promoting Neovascularization

This research was aimed to investigate the therapeutic effects of acupuncture treatment (APT) on experimental rats with intracerebral hemorrhage (ICH) and its potential mechanisms. An ICH animal model was established by infusion of autologous blood. Then, the local temperature, blood flow volume, nitric oxide (NO) and carbon monoxide (CO) contents in tissues surrounding hematoma were determined at 3 and 7 days after ICH model prepared. Furthermore, the mRNA and proteins expressions of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (b-FGF), angiogenin (ANG) I, tissue type plasminogen activator (t-PA) and plasminogen activator inhibitor (PAI) were determined by using real-time polymerase chain reaction analysis (RT-PCR) and immunohistochemical assays. Our results showed that APT increased the local temperature (P<0.05), blood flow volume (P<0.05), and contents of NO (P<0.05) and CO (P<0.05) at both day 3 and 7, compared with the control rats. In addition, from the RT-PCR and immunohistochemical assays, APT also up-regulated the mRNA and proteins expressions of VEGF (P<0.05), b-FGF (P<0.05), ANG I (P<0.05) and t-PA (P<0.05) in brain tissues surrounding hematoma whereas the PAI (P<0.05) down-regulated compared with normal rats at both 3 and 7 days. Collectively, our present results indicated that APT possesses notable therapeutic effects on experimental ICH rats via promoting neovascularization.