Experimental Study On The Construction Of Tissue Engineering Bone With Autogenous Oxygen-Emitting Nano-Bionic Scaffolds And Mir-142-5p-Guided Bone Marrow Mesenchymal Stem Cells

Autogenous oxygen-releasing nano-bionic scaffolds can promote Bone marrow mesenchymal stem cells (BMSCs) differentiation. Mir-142-5p has not been reported in regulation of BMSCs differentiation. This study intends to investigate the role of autogenous oxygen release nano-bionic scaffolds combined with Mir-142-5p-induced BMSCs in constructing tissue engineered bone. Rat BMSCs were isolated and transfected with Mir-142-5p scramble (negative control group) and Mir-142-5p mimics (miR-106a mimics group). Healthy male SD rats were assigned into fracture group; negative control group and Mir-142-5p mimics group which was implanted with autologous oxygen release nano-bionic scaffolds and transfected with Mir-142-5p-induced BMSCs followed by analysis of epiphysis growth, ALP activity, BMP-9 expression by ELISA and type II collagen expression by Real time PCR. Autogenous oxygen-releasing nano-bionic scaffold combined with BMSCs significantly promoted local bone density increase in epiphysis, promoted epiphyseal healing and ALP secretion, increased expression of type II collagen and EIMP-9 compared to fracture group (P < 0.05), in which autogenous oxygen-releasing nano-bionic scaffolds combined with Mir-142-5p-induced BMSCs had more significant effects on bone repair. BMSCs induced by autogenous oxygen-releasing nanobionic scaffolds combined with Mir-142-5p can regulate BMP-9, increase osteogenic transformation at bone defects, and promote bone healing.