Paralysiella testudinis gen. nov., sp. nov., isolated from the cloaca of a toad-headed turtle (Mesoclemmys nasuta)

A bacterial strain designated 26B(T), which had been isolated from the cloaca of a toad-headed turtle, was subjected to a comprehensive taxonomic study. Comparison of 16S rRNA gene sequences demonstrated that strain 26B(T) is a member of the family Neisseriaceae. Based on highest similarity values, Neisseria animaloris DSM 21642(T) (95.15%), Alysiella filiformis ATCC 15532(T) (95.06%), Uruburuella testudinis 07_0D624(-) (94.71 %), Uruburuella suis CCUG 47806(T) (94.66%) and Alysiella crassa DSM 2578(T) (94.64%) were identified as the closest relatives. Average nucleotide identity values based on the BLAST algorithm (ANIb) indicated that U. suis (76.10/76.17%), Neisseria shayeganii 871(T) (74.34/74.51 %), Stenoxybacter acetivorans (73.30/73.41 %), N. animaloris (72.98/72.80)%, A. filiformis (71.14/71.21 %) and A. crassa (70.53/71.15%) are the next closest relatives. Like ANIb, genome-based phylogeny did not suggest the affiliation of strain 26B(T) with any established genus. The polyamine pattern consisted of the major compounds putrescine, 1,3-diaminopropane and spermidine and the major quinone was ubiquinone Q-8. In the polar lipid profile, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an ornithine lipid were predominant. The fatty acid profile contained predominantly C-16:1 omega 7c, C-12:0, C-14:0, C-16:0 and C-12:0 30H. The size of the genome was 2.91 Mbp and the genomic G+C content was 54.0 mol%. Since these data do not demonstrate an unambiguous association with any established genus, we here propose the novel genus Paralysiella with the type species Paralysiella testudinis gen. nov., sp. nov. The type strain is 26B(T) (=CCM 9137(T)=LMG 32212(T)).