Highly resolved spatial transcriptomics for detection of rare events in cells
biorxiv(2021)
Abstract
Single-cell spatial transcriptomics technologies leveraged the potential to transcriptionally landscape sophisticated reactions in cells. Current methods to delineate such complex interplay lack the flexibility in rapid target adaptation and are particularly restricted in detecting rare transcripts. We developed a multiplex single-cell RNA In-situ hybridization technique, called ‘Molecular Cartography’ (MC) that can be easily tailored to specific applications and, by providing unprecedented sensitivity, specificity and resolution, is particularly suitable in tracing rare events at a subcellular level. Using a SARS-CoV-2 infection model, MC allows the discernment of single events in host-pathogen interactions, dissects primary from secondary responses, and illustrates differences in antiviral signaling pathways affected by SARS-CoV-2, simultaneously in various cell types.
### Competing Interest Statement
S.G., D.P., E.F.-H., M.H. and K.Z. declare no competing financial interests. C.F., A.M., A.B., C.U., B.N., S.S., A.G., F.R. and C.K. are Resolve BioSciences employees.
MoreTranslated text
Key words
spatial transcriptomics,rare events,cells
AI Read Science
Must-Reading Tree
Example
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined