NFIC regulates ribosomal biology and ER stress in pancreatic acinar cells and suppresses PDAC initiation
biorxiv(2021)
摘要
Tissue-specific differentiation is driven by specialized transcriptional networks. Pancreatic acinar cells crucially rely on the PTF1 complex, and on additional transcription factors, to deploy their transcriptional program. Here, we identify NFIC as a novel regulator of acinar differentiation using a variety of methodological strategies. NFIC binding sites are found at very short distances from NR5A2-bound genomic regions and both proteins co-occur in the same complex. Nfic knockout mice show reduced expression of acinar genes and, in ChIP-seq experiments, NFIC binds the promoters of acinar genes. In addition, NFIC binds to the promoter of, and regulates, genes involved in RNA and protein metabolism; in Nfic knockout mice, p-RS6K1 and p-IEF4E are down-regulated indicating reduced activity of the mTOR pathway. In 266-6 acinar cells, NFIC dampens the ER stress program through its binding to ER stress gene promoters and is required for complete resolution of Tunicamycin-mediated ER stress. Normal human pancreata from subjects with low NFIC mRNA levels display reduced epxression of genes down-regulated in Nfic knockout mice. Consistently, NFIC displays reduced expression upon induced acute pancreatitis and is required for proper recovery after damage. Finally, expression of NFIC is lower in samples of mouse and human pancreatic ductal adenocarcinoma and Nfic knockout mice develop an increased number of mutant Kras -driven pre-neoplastic lesions.
### Competing Interest Statement
The authors have declared no competing interest.
* ChIP
: chromatin immunoprecipitation
DEG
: differentially expressed genes
EMT
: epithelial-mesenchymal transition
ER
: endoplasmic reticulum
GSEA
: Gene set enrichment analysis
IF
: immunofluorescence
IHC
: immunohistochemistry
PDAC
: pancreatic ductal adenocarcinoma
TF
: transcription factor
TM
: tunicamycin
UPR
: unfolded protein response
更多查看译文
关键词
pancreatic acinar cells,ribosomal biology
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要