Highly multiplexed imaging of biosensors in live cells
bioRxiv (Cold Spring Harbor Laboratory)(2020)
摘要
Fluorescent biosensors allow for real-time monitoring of biochemical activities in cells, but their multiplexing capacity is severely limited by the availability of spectral space. We overcome this problem by developing a set of barcoding proteins that are spectrally separable from commonly used FRET (fluorescence resonance energy transfer)-based and single-fluorophore biosensors. Mixed populations of barcoded cells expressing different biosensors can be concurrently imaged and computationally unmixed to achieve highly multiplexed tracking of biochemical activities in live cells.
### Competing Interest Statement
The authors have declared no competing interest.
更多查看译文
关键词
multiplexed imaging,biosensors,cells
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要