Target lipidomics reveals associations between serum sphingolipids and insulin sensitivity by the glucose clamp
biorxiv(2020)
摘要
Background This study aimed to systematically investigate the associations between serum sphingolipids and insulin sensitivity as well as insulin secretion. This study also aimed to reveal potential predictors for insulin sensitivity or give perceptive insight into disease processes.
Methods We conducted a lipidomics evaluation of molecularly distinct SPs in the serum of 86 consecutive Chinese adults with or without obesity and diabetes using electrospray ionization mass spectrometry coupled with liquid chromatography. The GIR30 was measured under steady conditions to assess insulin sensitivity by the gold standard hyperinsulinemic-euglycemic clamp. We created the ROC curves to detect the serum SMs diagnostic value and establish the diagnosis of insulin sensitivity.
Results Differential correlation network analysis illustrated correlations amongst lipids, insulin sensitivity, insulin secretion and other clinical indexes. Total and subspecies of serum SMs and globotriaosylceramides (Gb3s) were positively related to GIR30, free FAs (FFA 16:1, FFA20:4), some long chain GM3 and complex ceramide GluCers showed strong negative correlations with GIR30. Notably, ROC curves showed that SM/Cer and SM d18:0/26:0 may be good serum lipid predictors of diagnostic indicators of insulin sensitivity close to conventional clinical indexes such as 1/HOMA-IR (all areas under the curve >0.80) based on GIR30 as standard diagnostic criteria.
Conclusions These results provide novel associations between serum sphingolipid between insulin sensitivity measured by the hyperinsulinemic-euglycemic clamp. We further identify two specific SPs that may represent prognostic biomarkers for insulin sensitivity.
* SP
: sphingolipid
SM
: sphingomyelin
GM3
: sphingosine
FAs
: fatty acids
GluCers
: Glu-ceramides
LacCers
: Lac-ceramides
GIR30
: glucose infusion rate over 30 minutes
FPIR
: first-phase insulin release
IVGTT
: intravenous glucose tolerance test
ROC
: receiver operating characteristic
IR
: insulin resistance
LC-MS
: liquid chromatography-mass spectrometry
QUICKI
: quantitative insulin sensitivity check index
GDR
: glucose disposal
M
: metabolizable glucose
TC
: total cholesterol
TG
: triglyceride
LDL-c
: LDL-cholesterol
HDL-c
: HDL-cholesterol
ALT
: alanine transaminase
AST
: aspartate transaminase
Gb3
: globotriaosylceramides
CerS
: ceramide synthase
SM/Cer
: ratio of total serum SMs and Cers.
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关键词
serum sphingolipids,insulin sensitivity,glucose
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