Rapid diagnostic detection of tomato apical stunt viroid based on isothermal reverse transcription-recombinase polymerase amplification
Journal of Virological Methods(2022)
Abstract
•Two RT-RPA assays using AmplifyRP® platform for TASVd detection were developed.•Both RT-RPA assays possess high specificity and sensitivity.•Crude extract decreased sensitivity of AmplifyRP® Acceler8 and AmplifyRP® XRT.•Developed RT-RPA assays can be used in laboratory and field conditions.
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Key words
RT-RPA,AmplifyRP® Acceler8™,AmplifyRP® XRT,Tomato apical stunt viroid,Viroid detection
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