The effect of 4-hexylresorinol administration on NAD+ level and SIRT activity in Saos-2 cells

Background 4-hexylresorcinol (4HR) has been shown to have anti-oxidant activity similar to that of resveratrol. As resveratrol increases sirtuin (SIRT) activity, 4HR might behave similarly to resveratrol. Method In this study, the expression levels of SIRT1, SIRT3, and SIRT6 were evaluated after 4HR administration (1–100 μM). As NAD+ is a substrate for SIRTs, its levels with SIRT activity were also studied. Results In the results, SIRT3 (100 μM at 24 h) and SIRT6 (1–100 μM at 24 h and 10 μM at 8 h) were shown to have significantly higher expression levels compared to untreated control ( p < 0.05). Pan-SIRT activity and the NAD+ level was significantly increased compared to that of the untreated control ( p < 0.05; 10 and 100 μM at 24 h). Conclusion 4HR administration increased SIRT activity and the NAD+ level in Saos-2 cells.