The potential of Zishen Yutai pills to facilitate endometrial recovery and restore fertility after induced abortion in rats

Context Abortions damage the endometrium in women. Currently, therapeutic options for endometrial recovery are limited. Zishen Yutai Pill (ZYP) was found to promote endometrial blood supply as a traditional Chinese medicine. However, whether ZYP promotes endometrial recovery post-abortion has not yet been explored. Objective This study evaluated the role of ZYP in rat endometrial recovery after induced abortion and explored its mechanism of action. Materials and methods Sprague-Dawley rats were divided into three groups: no-operation group, control group, and ZYP group. The rats in the control and ZYP group were induced abortion, and then treated with normal saline or ZYPs, respectively, for 1-3 oestrous cycles. Morphological changes in the endometrium were examined. Expression levels of the factors related to endometrial recovery were analyzed. The duration of this study was almost seven months. Results The endometrial thickness (7.3 +/- 0.17 mm) and number of glands (5.5 +/- 0.20) increased significantly in the ZYP group compared with those in the control group (5.5 +/- 0.15 mm and 3.5 +/- 0.18; p < 0.05). Fibrosis of the endometrium was ameliorated by ZYP administration (45 +/- 6% vs. 58 +/- 7%; p < 0.05). ZYPs treatment increased the expression of VEGF, ER, MMP-9, LIF, and HB-EGF, but decreased TGF-beta expression. Moreover, the average number of pups in the ZYP group (9.0 +/- 1.5) was greater than that in the control (4 +/- 1.3). Discussion and conclusion ZYPs accelerate endometrial recovery and restored fertility in rats, suggesting its potential to promote human endometrial repair.