Roseomonas oleicola sp. nov., isolated from an oil production mixture in Yumen Oilfield, and emended description of Roseomonas frigidaquae

A pink, ovoid- shaped, Gram- stain- negative, strictly aerobic and motile bacterial strain, designated ROY- 5- 3T, was isolated from an oil production mixture from Yumen Oilfield in PR China. The strain grew at 4-42 degrees C (optimum, 30 degrees C), at pH 5-10 (optimum, 7) and with 0-5 % (w/v) NaCl (optimum, 0%). The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that ROY- 5- 3T belongs to the genus Roseomonas and shared the highest pairwise similarities with Roseomonas frigidaquae CW67T (98.1%), Roseomonas selenitidurans BU-1T (97.8%), Roseomonas tokyonensis K-20T (97.7%) and Roseomonas stagni HS-69T (97.3%). The average nucleotide identity and digital DNA-DNA hybridization values between ROY- 5- 3T and other related type strains of Roseomonas species were less than 84.08 and 28.60 %, respectively, both below the species delineation threshold. Pan- genomic analysis showed that the novel isolate ROY- 5- 3T shared 3265 core gene families with the four closely related type strains in Roseomonas, and the number of strain- specific gene families was 513. The major fatty acids were identified as summed feature 8 (C18 : 1co6c/C18 : 1co7c), summed feature 3 (C16 : 1co6c/C16 : 1co7c) and C16 : 0. Strain ROY-5-3T contained Q- 10 as the main ubiquinone and the genomic DNA G+C content was 69.8 mol%. The major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol. Based on the phylogenetic, morphological, physiological, chemotaxonomic and genome analyses, strain ROY- 5- 3T represents a novel species of the genus Roseomonas for which the name Roseomonas oleicola sp. nov. is proposed. The type strain is ROY- 5- 3T (=CGMCC 1.13459T =KCTC 82484T).