Comparison of Multiplex Real-Time PCR and Droplet Digital PCR for Detection of Plasma Epstein-Barr Virus DNA in Nasopharyngeal Carcinoma

J A Miller,C Huang,M K Sahoo,H Wang,Q T Le,B Pinsky

INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS（2021）

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摘要

A multiplex ddPCR amplifying single- and variable-copy EBV targets in plasma offers similar analytical performance as the existing NRG-HN001 qPCR assay without the need for external calibrators. Further research will inform whether inter-laboratory precision is superior with ddPCR, which may facilitate standardization of EBV DNA quantitation.

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