Evidence of co-amplification of microbial DNA affecting interpretation at the D16S539 locus in the PowerPlex® ESI 17 Fast kit?: A case report

Forensic Science International: Genetics（2022）

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Abstract

•Recovery of DNA from damp/mouldy items during routine forensic casework.•DNA analysis using the Powerplex ESI-17 Fast kit.•PCR artefact at D16S539 locus masquerading as a ‘15′ allele.•Potential misinterpretation of genotype.•Probably caused by co-amplification of co-extracting microbial DNA.

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