Predictive value of miR-374a-5p of peripheral blood mononuclear cells in deep venous thrombosis for elderly patients after total hip arthroplasty

Objective: This study analyzed the predictive value of peripheral blood mononuclear cells' (PBMCs) miR-374a-5p in deep vein thrombosis (DVT) after total hip arthroplasty in the elderly. Methods: There was a total of 112 elderly patients that underwent elective total hip arthroplasty in our hospital and were enrolled as the research subjects. According to the color Doppler ultrasound of lower limbs 5 d postoperatively, subjectswere classified into thrombosis group (n=31) and non-thrombosis group (n=81). We detected the expression levels of miR-374a-5p in PBMCs and plasma D-dimer of the two groups of patients 1 d before surgery, and 1 d, 3 d, and 5 d after surgery, and analyzed the predictive value, and the correlation between miR-374a-5p and plasma D-dimer for DVT by ROC curve. Results: The relative expression of miR-374a-5p in PBMCs and plasma D-dimer of the two groups 1 d, 3 d, and 5 d after surgery were higher than those on the 1 d before surgery (P<0.05), and the indexes of thrombusgroup-patients were higher than those in the non-thrombosis group at various time points after surgery (P<0.05). We analyzed the predictive value of miR-374a-5p for DVT by ROC curve, and the areas under the predictive curve of miR-374a-5p on 1 d pre-surgery, and 1 d, 3 d and 5 d post-surgery for DVT were 0.435, 0.820, 0.974 and 0.782 respectively. The areas under the predictive curve of D-dimer for DVT was 0.470, 0.834, 0.863 and 0.790 respectively on 1 d pre-surgery, and 1 d, 3 d and 5 d post-surgery. The correlation analysis indicated that miR-374a-5p was extremely positively correlated with D-dimer (P<0.05). Conclusion: miR-374a-5p is of important value in the prediction of DVT in elderly patients with total hip arthroplasty. Its expression level has a critically positive correlation with D-dimer degree, which is likely to be abiologic indicator for the early diagnosis and treatment of DVT.