A broad and potent IgM antibody against tetra-EV-As induced by EVA71 and CVA16 co-immunization

Objective: To determine the potent and broad neutralizing monoclonal antibody (mAb) against entero-virus A (EV-A) in vitro and in vivo induced by enterovirus A71(EVA71) and coxsackievirus 16 (CVA16) co-immunization. Methods: The mAb was Generated by co-immunization with EVA71 and CVA16 through hybridomas technology. The characteristics and neutralizing ability of mAb were analysed in vitro and in mice. Results: We screened three mAb, the IgM antibody M20 and IgG antibody B1 and C31. All three antibod-ies showed cross-reactivity against tetra-EV-As. However, M20 showed potent and broad neutralizing ability against tetra-EV-As than B1 and C31. Meanwhile, M20 provided cross-antiviral efficacy in tetra -EV-As orally infected mice. Moreover, M20 binds to a conserved neutralizing epitope within the GH loop of tetra-EV-As VP1. Conclusions: M20 and its property exhibited potent and broad antiviral activity against tetra-EV-As, and that is expected to be a potential preventive and therapeutic candidate against EV-As. (c) 2021 Elsevier Ltd. All rights reserved.