Effects of Bacillus lipopeptides on the survival and behavior of the rosy apple aphid Dysaphis plantaginea

Within the framework of biocontrol development, several natural lipopeptides produced by Bacillus subtilis show well-documented anti-microbial properties, especially in orchards. However, the number of studies on their putative insecticidal effects remain low despite the growing interest to develop new strategies of orchards pests' control. The rosy apple aphid Dysaphis plantaginea is the major aphid pest causing great leaf damage to apple trees. In this study, we submitted young adult aphids to topical application of three different families of lipopeptides, Plipastatin (Fengycin), Mycosubtilin (Iturin), and Surfactin, either separately or as a ternary mixture. Their aphicidal effects were investigated at 1, 2.5 and 5 g/L, both at 1 h and 24 h after exposure, and their effects on aphid behavior were studied at the 2.5 g/L concentration at 24 h after exposure. When delivered alone, lipopeptides displayed contrasted effects varying from no aphicidal activity for Mycosubtilin to a mortality induced even at low concentrations by Surfactin. Surprisingly, locomotor activity of the surviving aphids was only affected by the two least lethal treatments, Mycosubtilin and the ternary mix. Their feeding behavior was only impacted by Surfactin, the most lethal treatment, that unexpectedly increased phloem sap ingestion. The results are discussed in the context of lipopeptides applicability for integrated pest management.