Activated gut-homing CD8 + T cells for coeliac disease diagnosis on a gluten-free diet

BMC MEDICINE(2021)

引用 4|浏览18
暂无评分
摘要
Background The diagnosis of coeliac disease (CD) in individuals that have started a gluten-free diet (GFD) without an adequate previous diagnostic work-out is a challenge. Several immunological assays such as IFN-γ ELISPOT have been developed to avoid the need of prolonged gluten challenge to induce the intestinal damage. We aimed to evaluate the diagnostic accuracy of activated gut-homing CD8 + and TCRγδ + T cells in blood after a 3-day gluten challenge and to compare it with the performance of IFN-γ ELISPOT in a HLA-DQ2.5 subsample. Methods A total of 22 CD patients and 48 non-CD subjects, all of them following a GFD, underwent a 3-day 10-g gluten challenge. The percentage of two T cell subsets (CD8 + CD103 + β7 hi CD38 + /total CD8 + and TCRγδ + CD103 + β7 hi CD38 + /total TCRγδ + ) in fresh peripheral blood drawn baseline and 6 days after the challenge was determined by flow cytometry. IFN-γ ELISPOT assays were also performed in HLA-DQ2.5 participants. ROC curve analysis was used to assess the diagnostic performance of the CD8 + T cell response and IFN-γ ELISPOT. Results Significant differences between the percentage of the two studied subsets of CD8 + and TCRγδ + cells at days 0 and 6 were found only when considering CD patients ( p < 10 −3 vs. non-CD subjects). Measuring activated CD8 + T cells provided accurate CD diagnosis with 95% specificity and 97% sensitivity, offering similar results than IFN-γ ELISPOT. Conclusions The results provide a highly accurate blood test for CD diagnosis in patients on a GFD of easy implementation in daily clinical practice.
更多
查看译文
关键词
CD8 T cells, Celiac disease, Gluten challenge, Gluten-free diet, IFN-gamma ELISPOT, Flow cytometry
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要