Multi-wavelength UV-based PAT tool for measuring protein concentration
Journal of Pharmaceutical and Biomedical Analysis(2022)
摘要
•A novel PAT tool has been proposed for online dilution free concentration estimation of IgG.•Validated for concentration range 0.8–100 g/L using feature engineering to circumvent saturation at higher concentrations•Application in enabling continuous chromatography/integrated operations demonstrated.•A low-cost, easy to implement tool which uses multi-wavelength UV data•Tested for samples from capture to formulation in a typical downstream train with< 8% variability.
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关键词
Protein quantification,Ultraviolet (UV) spectra,PLS,Chemometrics,Process analytical technology (PAT),Continuous chromatography
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