Development and validation of assays for the quantification of β-D-N4-hydroxycytidine in human plasma and β-D-N4-hydroxycytidine-triphosphate in peripheral blood mononuclear cell lysates

•Development of LC-MS/MS methods for NHC and anabolite quantification.•Validation of LC-MS/MS assays following FDA guidelines.•Pre-analytical considerations for accurate measurements of NHC and its anabolite.