Characterization of a macroporous epoxy-polymer based resin for the ion-exchange chromatography of therapeutic proteins

•Macroporous epoxy-based resins have been characterized.•Equilibrium binding capacity for therapeutic proteins was reached in a short time.•The large pore of resins improved the penetration of therapeutic proteins.•Efficient separation of therapeutic proteins was achieved at high linear velocity.•Resolution of proteins mixture was successful at high linear velocity.