The pMTL70000 modular, plasmid vector series for strain engineering in Cupriavidus necator H16

Muhammad Ehsaan,Jonathan Baker,Katalin Kovács,Naglis Malys,Nigel P Minton

Journal of Microbiological Methods（2021）

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摘要

Cupriavidus necator H16 can convert CO2 into industrial chemicals and fuels. To facilitate its engineering, we designed, built and tested the pMTL70000 modular plasmids comprising standardised Cupriavidus and E. coli replicons, selectable markers and application specific modules. Plasmids were characterised in terms of transmissibility, stability, copy number and compatibility.

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关键词

Cupriavidus necator H16,Modular vector,pMTL70000 series,Transformation,Segregational stability,Plasmid copy number

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