Characterization of the metabolite of cabozantinib generated from liver microsomes and hepatocytes by ultra-high performance liquid chromatography coupled to quadrupole/orbitrap high resolution mass spectrometry

•In vitro metabolism of cabozantinib was investigated.•26 metabolites including 15 new metabolites were identified.•CYP 3A4 was the major enzyme responsible for cabozantinib metabolism.•Oxidative defluorination, demethylation, hydroxylation and N-oxygenation were the major metabolites.