A Surface Plasmon Resonance-Based Assay for Simultaneous Measurement of Serum Concentrations of Therapeutic Antibodies and Anti-Drug Antibodies.
Methods in molecular biology (Clifton, N.J.)(2022)
摘要
The monitoring of the blood levels of therapeutic antibodies and their immune responses is proposed to guide and optimize therapy with these expensive drugs. We describe a novel Surface Plasmon Resonance (SPR)-based assay suitable for the simultaneous determination of serum concentrations of infliximab and anti-infliximab antibodies. The real-time detection by SPR avoids the incubation/washing steps of commonly used methods, thus allowing faster and more reliable measurements, in particular for low-affinity anti-drug antibodies. This method proved to be highly reproducible and may be well applied to other biotherapeutics.
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关键词
Anti-drug antibodies,Immunoassay,Real-time detection,Surface Plasmon Resonance,Therapeutic antibodies,Therapeutic drug monitoring
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