Sphingomonas Aliaeris Sp. Nov., A New Species Isolated From Pork Steak Packed Under Modified Atmosphere

Species belonging to the genus Sphingomonas have been isolated from environments such as soil, water and plant tissues. Many strains are known for their capability of degrading aromatic molecules and producing extracellular polymers. A Gram-stain-negative, strictly aerobic, motile, red-pigmented, oxidase-negative, catalase-positive, rod-shaped strain, designated DH-S5(T), has been isolated from pork steak packed under CO2-enriched modified atmosphere. Cell diameters were 1.5x0.9 mu m. Growth optima were at 30 degrees C and at pH 6.0. Phylogenetic analyses based on both complete 16S rRNA gene sequence and whole-genome sequence data revealed that strain DH-S5(T) belongs to the genus Sphingomonas, being closely related to Sphingomonas alpina DSM 22537(T) (97.4% gene sequence similarity), followed by Sphingomonas qilianensis X1(T) (97.4%) and Sphingomonas hylomeconis GZJT-2(T) (97.3%). The DNA G+C content was 64.4 mol%. The digital DNA-DNA hybridization value between the isolate strain and S. alpine DSM 22537(T) was 21.0% with an average nucleotide identity value of 77.03%. Strain DH-S5(T) contained 0-10 as the ubiquinone and major fatty acids were C-18:1 cis 11 (39.3%) and C-16(:1) cis 9 (12.5%), as well as C-1(6:)0 (12.1%) and C-14:0 2-OH (11.4%). As for polar lipids, phosphatidylcholine. phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, dimethylphosphatidylethanolamine and sphingoglycolipid could be detected, alongside traces of monomethylphosphatidylethanolamine. Based on its phenotypic, chemotaxonomic and phylogenetic characteristics, strain DH-S5(T) (=DSM 110829(T)=LMG 31606(T)) is classified as a representative of the genus Sphingomonas, for which the name Sphingomonas aliaeris sp. nov. is proposed.